/ Fractogel® EMD SO3 (S), (M). -. Ion Exchange chromatography using strong cation exchangers. Fractogel® ion exchangers are cross-linked. Fractogel® presents a range of media with “tentacular” geometries which provide an enhancement of surface topology that give the resins advantageous. Comparison between Merck’s Fractogel EMD DEAE and Sigma’s DEAE- Sepharose weak anion exchanger for ion exchange chromatography. I am purifying an.
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Chun-hua Cai 2 Estimated H-index: Results of this paper may be used in the selection of resins for further testing in biopharmaceutical protein purification process development.
Marina UrmannHeiner Graalfs.
Influence of surface modification on protein retention in ion-exchange chromatography: Jerker Porath 49 Estimated H-index: Packing of Toyopearl columns for gel filtration: Daniel Magiera 2 Estimated H-index: Publisher Summary Size exclusion chromatography SEC fractoge the predominant chromatographic technique for determining the molecular weight distribution of macromolecules. Are you looking for Influence of packing velocity on column performance.
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Particle size distribution was determined by two independent methods, coulter counting and SEM pictures, with fair agreement. Dependence of optimal packing velocity on column size.
Cation-exchange chromatography of monoclonal antibodies: Resins with good pure aprotinin capacity also performed well for aprotinin in fermentation broth, but the highest relative capacity was obtained with MacroPrep High S having a fairly low pure component dynamic capacity. Characterisation of a novel stationary phase designed for production-scale purification mabs [IF: John Westerlund 1 Estimated H-index: Yoshio Kato 19 Estimated H-index: For lysozyme and aprotinin with pI above experimental pH, dependence of pH on retention was generally low, though some pronounced decrease of retention with increasing pH was observed for CM HyperZ.
Djuro Josic 27 Estimated H-index: Other Papers By First Author. Evaluation using different retention models journal of chromatography a [IF: New ion exchangers for the chromatography of biopolymers.
Earle Stellwagen 33 Estimated H-index: Strong and weak cation-exchangers were compared for a number of chromatographic parameters, i.
Size-exclusion chromatography of plasma proteins with high molecular masses. Elution of lipoprotein fractions containing apolipoproteins E and A-I in size exclusion on Superose 6 columns is sensitive to mobile phase pH and ionic strength.
One main advantage of SEC is that: Testing was done with three proteins: Unger University of MainzM.
Determination of alkaline phosphatase aggregation by size exclusion high-performance liquid chromatography with low-angle laser light scattering detection. Chromatography Methacrylate Molar mass distribution Organic fracrogel Ionic strength Materials science Size-exclusion chromatography Macromolecule Polymer Agarose Glycidyl methacrylate.
Thus, gels made for SEC should have controlled range of size of the pores. Binding strength data of cation-exchange resins as a function of ionic strength depends fraactogel the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins.
Fractogel emd deae ml LABWARE Generic Labware
Montelaro Louisiana State University. The effect of competitive binding from yeast fermentation components on dynamic binding capacity of aprotinin was studied showing a significant decrease in binding capacity. Chatziathanassiou University of Mainz. View in Source Cite this fmd. The most suited and widely distributed packings consist of glycidyl methacrylate copolymers, agarose, or composite gels containing dextran.
Comparison of chromatographic ion-exchange resins V. Strong and weak cation-exchange resins.
Packing of toyopearl column for gel filtration: The methacrylate-based copolymer Fractogel EMD BioSEC used for large-scale purification of biological macromolecules is introduced to overcome ed drawback derived from the poor pressure stability of soft gels.
The separation mode is based on the differential penetration of molecules of different size and shape into the pores of the stationary phase. Efficiency results present the expected trend of increasing dependence of plate fracttogel as a function of increasing flow rate, and the highest flow dependence was observed for Fractogel EMD COO.